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AlleleID

Design TaqMan® Probes

  • TaqMan® probes: AlleleID designs optimal TaqMan® probes free of dimers, repeats and runs to ensure signal fidelity.

  • TaqMan® MGB probes: AlleleID supports the design of TaqMan® MGB probes which are typically shorter as compared to the regular TaqMan® probes

  • Multiplex & allele identification assays: AlleleID supports multiplexing for up to four sequences, avoids cross homologies with all dual labeled probes and primers preventing competition in multiplex reactions. Designs wild and mutant TaqMan® probes for SNP identification.

  • Evaluate pre-designed TaqMan® probes based assays: Pre-designed primer sets can be evaluated and a compatible TaqMan® probe designed. Similarly, a pre-designed TaqMan® probe can be evaluated and compatible primers designed. You can even import the primers designed in the SYBR® Green primer design mode and then design a compatible probe.

  • Graphical view: Displays a graphical view of TaqMan® probe secondary structures.

  • Ranking: Only the best TaqMan® probes are designed for each template, using statistical optimization technique.

  • Read about how TaqMan® probes work!
 
AlleleID Features AlleleID Highlights AlleleID Applications
-Real Time PCR Primer Design

-SYBR® Green Primer Design

-TaqMan® Probe Design

-Molecular Beacon Design

-FRET probe design

-Microarray Probe Design


-Database Support

-Input & Output Formats

-Cross Species/ Taxa Specific Assays

-Species Identification Assays

-Microarray Based Gene Splicing Detection

-Exon Junction Primer Design

-MLPA® Probe Design

-Pathogen Detection

-Bacterial Identification

-Gene Splicing

-Environment Monitoring

-Biodiversity Studies

-Biodefense Studies


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SYBR® is a registered trademark of Molecular Probes, Inc. | TaqMan® is a registered trademark of Roche Molecular Systems, Inc.
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