Exon Junction Primer Design

Using AlleleID®, you can also design primers across exon junctions. The exons are identified by interpreting GenBank header annotations or by specifying them manually.

To selectively amplify cDNA from a mixture of cDNA and gDNA, the primers are designed such that at least one primer from a pair spans an exon junction. The primers are designed to anneal to mRNA or cDNA synthesized from it but not to genomic DNA.