AlleleID

What's new in this version

Exon Junction Primer Design

Using AlleleID, you can also design primers across exon junctions. The exons are identified by interpreting GenBank header annotations or by specifying them manually.

To selectively amplify cDNA from a mixture of cDNA and gDNA, the primers are designed such that at least one primer from a pair spans an exon junction. The primers are designed to anneal to mRNA or cDNA synthesized from it but not to genomic DNA.