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Molecular Beacon Design

  • Tm adjustment: Automatically selects a stem of appropriate length for optimal beacon Tm.

  • Optimal beacons: Designs beacons free of dimers, repeats, and runs for increased signal strength.

  • Multiplex beacons & primers: Checks molecular beacons for cross homologies with all primers preventing competition in multiplex reactions.

  • Allele identification: Designs molecular beacons for detection of both wild and mutant alleles.

  • Tm calculation: Highly accurate hairpin Tm calculation.

  • Evaluate pre-designed molecular beacon assays: Pre-designed primer sets can be evaluated or primers for a pre-designed molecular probe can be constructed. Similarly, a pre-designed beacon can be evaluated or a beacon can be designed for a pre-designed primer set. This facilitates using SYBR® Green primers for beacon assays which can be used for bacterial identification assays or any other assay.

  • Lightning fast: Processes an average cDNA sequence in under a second.

  • Graphical view: Displays designed beacon graphically along with its properties.

Read about molecular beacons.

 
 
AlleleID® Features AlleleID® Highlights AlleleID® Applications
-Real Time PCR Primer Design

-SYBR® Green Primer Design

-TaqMan® Probe Design

-Molecular Beacon Design

-FRET probe design

-Microarray Probe Design


-Database Support

-Input & Output Formats

-Cross Species/ Taxa Specific Assays

-Species Identification Assays

-Microarray Based Gene Splicing Detection

-Exon Junction Primer Design

-MLPA® Probe Design

-Luminex xMAP® Assays
-Pathogen Detection

-Bacterial Identification

-Gene Splicing

-Environment Monitoring

-Biodiversity Studies

-Biodefense Studies

 
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