-
Tm adjustment: Automatically selects a stem of appropriate length for optimal beacon Tm.
-
Optimal beacons: Designs beacons free of dimers, repeats, and runs for increased signal strength.
-
Multiplex beacons & primers: Checks molecular beacons for cross homologies with all primers preventing competition in multiplex reactions.
-
Allele identification: Designs molecular beacons for detection of both wild and mutant alleles.
-
Tm calculation: Highly accurate hairpin Tm calculation by connecting to the Mfold server.
-
Evaluate pre-designed molecular beacon assays: Pre-designed primer sets can be evaluated or primers for a pre-designed molecular probe can be constructed. Similarly, a pre-designed beacon can be evaluated or a beacon can be designed for a pre-designed primer set. This facilitates using SYBR® Green primers for beacon assays which can be used for bacterial identification assays or any other assay.
-
Lightning fast: Processes an average cDNA sequence in under a second.
-
Graphical view: Displays designed beacon graphically along with its properties.
- Read about molecular beacons.
Play Online
