Molecular Beacon Design

• Tm adjustment: Automatically selects a stem of appropriate length for optimal beacon Tm.

• Optimal beacons: Designs beacons free of dimers, repeats, and runs for increased signal strength.

• Multiplex beacons & primers: Checks molecular beacons for cross homologies with all primers preventing competition in multiplex reactions.

• Allele identification: Designs molecular beacons for detection of both wild and mutant alleles.

• Tm calculation: Highly accurate hairpin Tm calculation.

• Evaluate pre-designed molecular beacon assays: Pre-designed primer sets can be evaluated or primers for a pre-designed molecular probe can be constructed. Similarly, a pre-designed beacon can be evaluated or a beacon can be designed for a pre-designed primer set. This facilitates using SYBR® Green primers for beacon assays which can be used for bacterial identification assays or any other assay.

• Lightning fast: Processes an average cDNA sequence in under a second.

• Graphical view: Displays designed beacon graphically along with its properties.

Read about molecular beacons.