Alternative Splicing
DNA microarray is perhaps the most useful technology for splice variant studies. AlleleID includes the ability to
design microarray probes for detecting alternative splicing and gene splicing events.
- AlleleID designs two types of microarray probes that we call junction probes and exon probes, giving the user complete control over the design parameters.
- AlleleID detects the alternative splicing events by designing probes for each junction and/or for each splice
variant created.
- AlleleID designs exon probes. Such probes are important to confirm the presence of an exon in the variant.
- Technical resource on Gene Splicing.
What is Alternative Splicing?
The coding and non-coding fragments of the gene can be arranged in different ways. When this produces different m-RNA sequences from the same parent gene, the phenomenon is known as Alternative Splicing. The biological consequences of this process can be severe as the same gene leads to formation of different proteins which may be functional, non-functional or malfunctioning. The varied forms of the splicing events are known as splice variants. And as outlines above, AlleleID could be used for splice variant detection using microarray experiments.
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