-
Probe design: You can design MLPA® probes, both for copy number detection and mutation studies. The software was developed in collaboration with MRC Holland, the inventors of the technology, and follows all MLPA design guidelines. AlleleID can also design custom oligos for Pam4U PamChip® Arrays.
-
Avoid Homology: AlleleID designs highly specific MLPA®probes by automatically avoiding the regions of homology identified during a BLAST search.
-
Template Secondary Structures: Template structures identified are avoided while designing MLPA® probes, making the reaction efficient.
-
Multiplex MLPA® Probes: Displays the free energies of the most stable cross-dimers formed between the MLPA® probes, helping you determine which probes are best suited for a multiplex reaction.
-
Load Mutations & SNPs: Allows specifying mutations such as insertions, deletions, substitutions and duplications in a sequence. Easily loads sequences containing SNPs from the variation descriptors in standard GenBank variation files.
-
Load Exons: Retrieves exon information from GenBank header annotations. If you are working with unpublished data you can easily add them.
- Sequence View: AlleleID displays the designed MLPA® probes and where they bind to the sequenc.
Play Online
