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An exceptional software to design cloning experiments and drawing publication quality plasmid vector maps

"I found SimVector to be the best program that I have used so far to produce publication quality vector graphics of eukaryotic nuclear gene models." -Dr. Jeffrey Moseley, Carnegie Institution of Washington, Stanford University, USA.

"...this package accommodates restriction enzyme-based cloning but also enables TA-based and Cre recombinase-based strategies."-The Scientist -full review

Feature Summary

  • Draw Accurate Plasmid Maps: SimVector is an exceptional tool to draw publication quality plasmid maps. The graphics can then be enhanced with patterns, styles, lines, and colors.

  • Vector Graphics: SimVector generates plasmid map images in vector graphic format for Adobe Illustrator 10 and Microsoft PowerPoint 2002.

  • Customize Vector Maps: Choose from hundreds of fonts, colors, styles and fill patterns for enzymes and features with a click.

  • Multiple Cloning Site (MCS) Display: SimVector identifies the MCS's by interpreting GenBank header annotations when available, or you can specify them manually. It even has the smarts to list only the enzymes that cut within the MCS and nowhere else. This special format we call the Bracket View.

    For displaying MCS's in a unique format, with enzymes one about the other in a vertical list, this feature is very useful for commercial companies.

  • Multiple Fonts: The program can now display any enzyme or feature name using multiple fonts. It is common to list an enzyme thus: Hind III, where the bacterium of origin is listed in italics, but not the number. Coupled with the MCS bracket view, SimVector now has all the functionality to draw plasmids and export them in several vector graphic or bitmap formats.

Design Cloning Experiments

  • Plan and Design Cloning Experiments: Design Gateway® Cloning, TA Cloning and Restriction cloning experiments.

  • Cloning Wizard: Use the intuitive interface to simulate cloning experiments to save time and laboratory resources.

  • Sequence Editing: Quickly and easily place inserts in vectors by simple cut and paste commands.

  • Active Analysis: Automatically updates restriction enzymes and features as a DNA sequence is edited for true WYSIWYG editing.

  • Sequence Notes: Keep printable notes of the designed cloning experiment for ready reference.

  • ORF Search: SimVector now includes the search capability to find ORFs in the chosen reading frames, identified with the selected start and stop codons, and compliant to the specified minimum length. With a click of a button, you can now ensure that the construct remains in-frame after cloning. The graphical ORF display is always ready for reference and further analysis.

  • Sequence Translation: Translate sequences for simulating expressed proteins and to check for possible reading frame errors.

Draw Exceptional Publication Quality Plasmid Maps

Whether you are a catalog company selling engineered plasmids or are performing restriction analysis for recombinant cloning experiments, SimVector will help you simulate the experiments and create publication quality plasmid maps from start to finish. Use the software to draw circular and linear plasmid maps in a variety of colors, patterns, fonts and line types. Display enzyme names in two font styles with the bacterium portion in Italics, as is traditional. Use even more than one font style and color in a single textual word anywhere in the plasmid map including in the feature annotations.

Export plasmid maps in EPS format for Adobe Illustrator 10, SVG for Microsoft Power Point 2002 or as ready-to-host web pages. Choose JPEG, TIFF and PNG formats for export.

Multiple Cloning Site (MCS) Display is Unique to SimVector

Vector catalogs generally display MCS's in a unique format, with enzymes one above the other in a vertical list. SimVector identifies the MCS's by interpreting GenBank header annotations or you can specify them manually. It then draws them in this special format we call the "Bracket View". It even has the smarts to list only the enzymes that cut within the MCS and nowhere else.



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