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TA Cloning

The TA cloning method takes advantage of the terminal transferase activity of some DNA polymerases such as Taq polymerase. This enzyme adds a single, 3'-A overhang to each end of the PCR product. This makes it possible to clone this PCR product directly into a linearized cloning vector with single, 3'-T overhangs. To clone such PCR product the linearized cloning vector with single, 3'-T overhangs called a T- vector are used. The PCR products with dA overhang, are mixed with this vector in high proportion. The complementary overhangs of "T" vector and PCR product will get hybridized and ultimate joining of two DNA fragments accomplished by T4 DNA ligase.

Perform TA Cloning with SimVector

SimVector automatically generates graphical DNA sequence maps of designed recombinant DNA molecules displaying vector - donor relationships and the corresponding cloning protocol. Use commercial T-vector or design your own T-vector using restriction digestion and recombine with the donor in sense or anti-sense orientation to generate highly accurate recombinant DNA sequence map.

TA Cloning: Design linearized commercial T Vector
TA cloning commercial T vector

TA Cloning: Design donor vector
TA cloning Doner vector

TA Cloning: Recombinant vector
TA Cloning Recombinent Vector

Draw Exceptional Publication Quality Plasmid Maps

Whether you are a catalog company selling engineered plasmids or are performing restriction analysis for recombinant cloning experiments, SimVector will help you simulate the experiments and create publication quality plasmid maps from start to finish. Use the software to draw circular and linear plasmid maps in a variety of colors, patterns, fonts and line types. Display enzyme names in two font styles with the bacterium portion in Italics, as is traditional. Use even more than one font style and color in a single textual word anywhere in the plasmid map including in the feature annotations.

Export plasmid maps in EPS format for Adobe Illustrator 10, SVG for Microsoft Power Point 2002 or as ready-to-host web pages. Choose JPEG, TIFF and PNG formats for export.

Multiple Cloning Site (MCS) Display is Unique to SimVector

Vector catalogs generally display MCS's in a unique format, with enzymes one above the other in a vertical list. SimVector identifies the MCS's by interpreting GenBank header annotations or you can specify them manually. It then draws them in this special format we call the "Bracket View". It even has the smarts to list only the enzymes that cut within the MCS and nowhere else.



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