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Protein Glycosylation

The addition of a carbohydrate moiety to a protein molecule is referred to as protein glycosylation. It is a common post translational modification for protein molecules involved in cell membrane formation. During this process,the linking of monosaccharide units to the amino acid chains sets up the stage for a series of enzymatic reactions that lead to the formation of glycoproteins (n and o linked oligosaccharides that are found to a protein entity). In all 16 known enzymes are supposed to mediate this reaction. A typical glycoprotein has at least 41 bonds which involve 8 amino acids and 13 different monosaccharide units and includes the glycophosphatidylinositol (GPI) and phosphoglycosyl linkages. Protein glycosylation helps in proper folding of proteins, stability and in cell to cell adhesion commonly needed by cells of the immune system. The major sites of protein glycosylation in the body are ER, Golgi body, nucleus and the cell fluid.

Protein glycosylation can be categorized in two main types:

N-Linked glycosylation in proteinsa) N-linked glycosylation: It begins with the addition of a 14-sugar precursor to an asparagine amino acid. It contains glucose, mannose and n-acetylglucosamine molecules. This entity is then transferred to the ER lumen. The oligosaccharyl transferase enzyme attaches the oligosaccharide chain to asparagine that occurs in the tripeptide sequence, Asn-X-Ser or Asn-X-Thr. X can be any amino acid other than Proline. The oligosaccharide attached protein sequence now folds correctly and is now translocated to the Golgi body where the mannose residue is removed.O-Linked glycosylation in proteins

b) O-linked glycosylation: Glycosylation begins with an enzyme mediated addition of N-acetyl-galactosamine followed by other carbohydrates to serine or threonine residues. Studies reveal that O linked glycosylation occurs at a later stage in protein processing.

An Innovative Glycan and Glycopeptide MSn Data Analysis Tool SimGlycan® predicts the structure of glycans and glycopeptides using mass spectrometry data.

SimGlycan® accepts the experimental MS/MS and Multi Stage/Sequential mass spectrometry (MSn, n>2) data, matches them with its own database of theoretical fragments and generates a list of probable candidate structures. Each structure is scored to reflect how closely it matches your experimental data. Apart from the structural information, other biological information for the probable molecular structures such as the glycan class (N-Linked, O-Linked heparin, lipopolysaccharide etc.), reaction, pathway and enzyme are also made available for easy reference in case of structural elucidation of glycans while in the case of glycopeptide qualitative analysis, information such as Protein ID, Protein Name, Source, Classification, Class, peptide sequence, peptide mass etc. are made available for identified glycopeptides.

Glycan & Glycopeptide MS/MS Data Analysis for Studying Glycosylation

Protein Glycosylation, which is a key post-translational modification, is the result of addition of a glycan to a peptide sequence. Glycopeptides are known to exhibit multiple biological functions. In order to identify distinct functional properties for defined structural features, detailed information on the respective glycan moieties is essential. In order to understand all these phenomena, glycosylation analysis is an area of growing interest. Glycans have also been found to participate in many biological processes including embryonic development, inter and intracellular activities, coordination of immune functions, pathogens homing on their host tissues, cell division processes and protein regulations and interactions.


  • Robust Glycan and Glycopeptide Database
  • Support for MALDI MS/MS and LC-MS/MS Workflows
  • Data Input File Formats
  • Accurate Ranking Mechanism
  • Additional Filters For Precise Results
  • Comprehensive Result Analysis
  • LC-MS and LC- MS/MS Data Processing
  • High Throughput Analysis
  • TMT Quantitation
  • Multi Stage Mass Spectrometry (MSn) Data Analysis
  • Glycopeptide Qualitative Analysis
  • Project Management
  • Draw Glycans
  • Mass Spectra Interpretation


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