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Citations and Reviews

Author and Details
Making the Most of Multiplex PCR Ellis, J.
Biocompare. 2018; November 15.

Exploring target-specific primer extension in combination with a bead-based suspension array for multiplexed detection and typing using Streptococcus suis as a model pathogen

Fimme J. van der Wal, René P. Achterberg, Conny van Solt-Smits, Jan H. W. Bergervoet, Marjanne de Weerdt, Henk J. Wisselink.
Journal of Veterinary Diagnostic Investigation. 2018; Jan; 30(1): 71–77.

A Survey of gyrA Target-Site Mutation and qnr Genes among Clinical Isolates of Escherichia coli in North of Iran

Setareh Yousefi, Ali Mojtahedi,Mohammad Shenagari.
Jundishapur Journal of Microbiology. 2018; In Press:e67293.

Heterozygosity analysis of polycystic kidney disease 1 gene microsatellite markers for linkage analysis of autosomal dominant polycystic kidney disease type 1 in the iranian population

Razieh Fatehi, Sharifeh Khosravi, Maryam Abedi, Rasoul Salehi, Yousof Gheisari.
Journal of Research in Medical Sciences. 2017; Sep 26;22:102. doi: 10.4103/jrms.JRMS_136_17.

Molecular dysexpression in gastric cancer revealed by integrated analysis of transcriptome data

Xiaomei Li, Weiwei Dong, Xueling Qu, Huixia Zhao, Shuo Wang, Yixin Hao, Qiuwen Li, Jianhua Zhu, Min Ye, Wenhua Xiao.
Oncology Letters. 2017; May;13(5):3177-3185. doi: 10.3892/ol.2017.5798.

Three multiplex snapshot assays for SNP genotyping in candidate innate immune genes

Lisa M Esteves, Sara M Bulhoes, Maria J Brilhante, Luisa Mota-Vieira.
BMC Research Notes. 2013; Feb 7;6:54. doi: 10.1186/1756-0500-6-54.

Public Health Value of Next-Generation DNA Sequencing of Enterohemorrhagic Escherichia coli Isolates from an Outbreak

Anthony P. Underwood, Tim Dallman, Nicholas R. Thomson, Michaela Williams, Katy Harker, Neil Perry, Bob Adak, Geraldine Willshaw, Tom Cheasty, Jonathan Green, Gordon Dougan, Julian Parkhill, John Wain.
Journal of Clinical Microbiology. 2013; Jan; 51(1): 232–237.doi: 10.1128/JCM.01696-12.

Evolution of Enterohemorrhagic Escherichia coli O26 Based on Single-Nucleotide Polymorphisms Stefan Bletz, Martina Bielaszewska, Shana R. Leopold, Robin Köck, Anika Witten, Jörg Schuldes, Wenlan Zhang, Helge Karch, Alexander Mellmann.
Genome Biology and Evolution. 2013;5(10):1807-16. doi: 10.1093/gbe/evt136.
Genome-wide single nucleotide polymorphism-based assay for high-resolution epidemiological analysis of the methicillin-resistant Staphylococcus aureus hospital clone EMRSA-15 A.Holmes, G.McAllister, P.R.McAdam, ShsienChoi, K.Girvan, A.Robb, G.Edwards, K.Templeton, J.R.Fitzgerald.
Clinical Microbiology and Infection 2014; Feb;20(2):O124-31. doi: 10.1111/1469-0691.12328.
Multiplex ARMS PCR to Detect 8 Common Mutations of ATP7B Gene in Patients With Wilson Disease Hassan Dastsooz, Mohammad Hadi Imanieh, Seyed Mohsen Dehghani, Mahmood Haghighat, Maryam Moini, Majid Fardaei.
Hepatitis Monthly. 2013; May 16;13(5):e8375. doi: 10.5812/hepatmon.8375.
Simultaneous Detection of Seven Enteric Viruses Associated with Acute Gastroenteritis by a Multiplexed Luminex-Based Assay Yan Liu, Zi-qian Xu, Qing Zhang, Miao Jin, Jie-mei Yu, Jin-song Li, Na Liu, Shu-xian Cui, Xiang-yu Kong, Hong Wang, Hui-ying Li, Wei-xia Cheng, Xue-jun Ma, Zhao-jun Duan.
Journal of Clinical Microbiology. 2012; Jul;50(7):2384-9. doi: 10.1128/JCM.06790-11.

A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A

Yajun Song, Philippe Roumagnac, François-Xavier Weill, John Wain, Christiane DolecekCamila J. Mazzoni, Kathryn E. Holt, Mark Achtman.
Journal of Antimicrobial Chemotherapy. 2010; Aug;65(8):1631-41. doi: 10.1093/jac/dkq175.


Oligo Design for Multiplex PCR & High Throughput SNP Genotyping and Analysis

PrimerPlex is an efficient and sophisticated tool for designing oligos for multiplex assays. Multiplex assays facilitate amplification of multiple targets in a single reaction vessel, reducing both the time and cost of experimentation.

Primer design for multiplex PCR presents several challenges which include primer dimers, inability to separate amplicons with similar electrophoretic mobility and mis-priming due to nonspecific binding to non-target DNA templates. PrimerPlex uses proprietary algorithms to design optimal multiplex primer sets under uniform reaction conditions for over 100 sequences. The primer sets are identified after screening all the primers in a pool and minimizing Tm mismatches to ensure specific amplification and high signal strength. In this process, it analyzes millions of possible multiplex primer sets in a few seconds and presents a list of alternate sets. You can specify minimum product size differences amongst the set of designed primer pairs for better visualization of bands on a gel. To assure primer specificity, primers can be BLAST searched from PrimerPlex against any of the genomic databases available at NCBI.


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