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NewsPREMIER Biosoft International today announced the world wide release of version 4 of AlleleID®The only tool that helps design microarrays and real time PCR diagnostic assays for pathogen detection or bacterial identification. The new version includes support for designing splice variant microarrays to detect gene splicing and alternative splicing events. Palo Alto, California--September 25, 2006, PREMIER Biosoft International today announced the world wide release of version 4 of AlleleID®, the only tool available for designing diagnostic assays for both real time PCR and microarrays. AlleleID® now includes support for designing probes for splice variant arrays. To detect the alternative splicing events, AlleleID® designs two types of probes called junction probes and intra-exon probes. Junction probes span exon-exon boundaries while intra-exon probes lie entirely within a single exon, making AlleleID® an effective tool in designing experiments to test novel splice forms. To design species identification assays, AlleleID® aligns sequences using ClustalW and analyzes conserved and species specific regions to design molecular probes for microarrays and TaqMan®/molecular beacon real time PCR assays. The assays are designed to detect only the strain or species of interest from the mix. For cross species assays, AlleleID® identifies conserved regions of related organisms that can be used with samples from any of the organisms. This powerful functionality can also be used for detection, identification, quantification of contaminants. |