PREMIER Biosoft International
combines the power of microarray based detection with
qPCR in the new version release of its pioneering
Palo Alto, CA-- December 19, 2005: PREMIER
Biosoft International releases version 2.00 of AlleleID®.
AlleleID® helps design microarrays and qPCR diagnostic
assays for rapid detection of pathogens or bacteria.
Typical applications include environmental monitoring,
in prevention/control of infectious diseases, development
of surveillance tests and biodiversity studies.
To design species identification assays, AlleleID®
aligns the sequences and analyzes species specific
regions to design probes for microarrays and primers
and probes for TaqMan®/molecular beacon qPCR assays.
The assays are designed to detect only the strain
or species of interest from the mix. For cross species
assays, AlleleID® identifies conserved regions of related
organisms to help study gene expression when genome
draft of the organism under study is not available.
It offers taxa specific designs for studies such as
effects of contaminants.
AlleleID® employs powerful and sophisticated algorithms
to design highly specific and efficient primers and
probes. It BLAST searches sequences and automatically
interprets the results to design specific oligos avoiding
regions of homology. The program incorporates an innovative
feature called "Minimal Set", which designs the fewest
number of oligos required for detection, lowering
The program also has extensive support for qPCR chemistries
such as SYBR® Green, TaqMan®, FRET probes
and beacons for allele discrimination, multiplex and
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