"I
wish I had analyzed my primers before
ordering for the third time. Now I check
all my primers with NetPrimer before
ordering" - Stacey McCann, Cancer Biology Program,
Stanford University
About NetPrimer
NetPrimer combines the latest primer
analysis algorithms with a web-based
interface allowing the user to analyze
primers over the Internet. All primers
are analyzed for primer melting temperature using the nearest neighbor thermodynamic
theory to ensure accurate Tm prediction.
Primers are analyzed for all primer secondary
structures including hairpins, self-dimers,
and cross-dimers in primer pairs.
This ensures the availability of
the primer for the reaction as well
as minimizing the formation of primer
dimer. The program eases quantitation
of primers by calculating primer
molecular weight and optical activity. To facilitate the selection of an
optimal primer, each primer is given
a rating based on the stability
of its secondary structures. A comprehensive
analysis report can be printed for
individual primers or primer pairs.
Major
Features
- Primer Melting Temperature (Tm): Predicts primer melting temperature using the accurate nearest neighbor thermodynamic
algorithm.
- Primer Properties: Tm , molecular
weight, GC%, optical activity
(both in nmol/A260 & µg/A260),
DG, 3' end stability, DH, DS,
and 5' end DG.
- Primer Secondary Structures:
Hairpins, dimers, cross dimers, palindromes,
repeats and runs.
- Primer Rating: Quantitative prediction of the
efficiency of a primer.
- Comprehensive Report:
Prints complete primer analysis for an individual
primer or primer pair
- Primer Pairs: Analyze
individual primers or primer pairs.
- Comprehensive Help: Details
all the formulas and references used in primer
analysis algorithm.
Our Other Products
AlleleID®: For designing qPCR assays and microarray experiments. Supports primer/probe design on nucleotide sequence alignments, exon junctions for bacterial identification, selective cDNA amplification and much more.
Array Designer: Design efficient and specific probes for SNP detection, SNP genotyping and microarray gene expression profiling.
Beacon Designer™: For designing real time PCR assays.
PrimerPlex: Multiplex SNP Genotyping oligo design tool for suspension array systems such as Luminex xMAP® technology.
Primer Premier: Comprehensive PCR primer design software.
SimGlycan®: For glycan analysis and structure prediction.
SimVector: Draw plasmid maps and simulate cloning experiments.
TMA foresight: For tissue microarray data analysis.
Xpression Primer: Tagged primers, sequencing primers, nested PCR for in-vitro expression analysis.
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