Real Time PCR Primer Design

Beacon Designer designs real time PCR primers that are optimized for the following chemistries viz. TaqMan®, molecular beacons and FRET. These real time PCR primers are specific, efficient and compatible with the probe that they work with:

• Primer Specificity: Primers are designed by avoiding significant cross homologies identified by automatically interpreting BLAST search results.
  
  
• Primer Efficiency: Primer efficiency is enhanced by automatically avoiding template structures identified by folding the template at MFold.
  
  
• Multiplex PCR Primer Design: Design support for multiplex assays is included. Up to four sequences in the TaqMan® design mode and five sequences in the molecular beacon design mode can be multiplexed.
  
  
• Use Pre-designed Primers: Include a well proven primer set for a template and design a compatible probe.