Beacon Designer™ supports the Scorpions® chemistry for real time detection of a target sequence. The Scorpions® primers designed are free from dimers, runs, repeats and have a melting temperature within a user specified range.
Beacon Designer™ automatically selects a stem of appropriate length for optimal Scorpions® Tm. You can design specific and efficient Scorpions® by automatically avoiding regions of significant cross homology and by avoiding regions on the template that are susceptible to folding. Beacon Designer™ graphically displays the alternate structures of the designed Scorpions® along with their properties. Alternate structures may lead to false positives or reduce the efficiency of the assay.
Using Beacon Designer™, you can also evaluate pre-designed Scorpions® assays. Pre-designed primer sets can be evaluated or primers for a pre-designed probe can be designed.