Design HRMA Primers
Beacon Designer™ offers a comprehensive solution for Mutation detection and SNP genotyping using High Resolution Melting Analysis (HRMA), a more cost-effective method than probe-based genotyping analysis. The program employs proprietary algorithms that enable designing the best High Resolution Melting Analysis primers for detecting all types of mutations including DIPs, MNPs, SNPs classes I and II for heterozygotes and homozygotes.
The primers are designed flanking the mutation of interest to generate the shortest possible amplicons with detectable melting temperature variation, taking into consideration all the parameters essential for High Resolution Melting Analysis. The primers are designed avoiding template secondary structures, assuring efficient primer extension. The designed primers can be BLAST searched against nucleotide databases, including the redundant nr database at NCBI, to verify their specificity.
You can use pre-designed or published or well proven forward or reverse primers and have Beacon Designer™ evaluate them for you. For example, for a given forward primer, Beacon Designer™ can design a reverse primer for HRMA assays.