tagged primer design tool for designing
expression cloning experiments
Use the sophisticated algorithm of Xpression Primer to design thousands of tagged primers for expression cloning systems such as Gateway®, BD In-Fusion™, epitope and TOPO® Tools. You can choose to amplify an entire ORF or generate N terminal or C terminal fusion proteins. Xpression Primer ensures that the reading frame of the amplified ORF is conserved. To work with other expression systems, simply add functional tags of your choice and design tagged primers.
Successful Amplification with Nested PCR
To ensure the success of your PCR experiment, let Xpression Primer design nested tagged primers to amplify ORFs. You can locate the outer primers anywhere in the UTRs or in regions of no significant homology. Xpression Primer will BLAST your sequences, automatically interpret the results and design highly specific primers. The tagged inner
primer pair amplifies the PCR product generated by the outer pair with little or no non-coding regions. You can also choose from a list of alternate primers to better meet specific experimental needs.
Sequencing for Product Verification Made Easy
The versatile algorithm of Xpression Primer can design optimal sequencing primers for multiple sequences in a single run. It picks forward primers across the amplicon at a
specified interval and reverse primers staggered on the opposite strand. You can export the results for several popular well plate configurations.
Generate Precise Transcripts
Design primers to generate sense or antisense transcripts for in vitro expression studies.
Xpression Primer searches Entrez and downloads batches of ORF sequences directly into the program.