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SimGlycan®

Application Notes

Title & Abstract Author & Publication

Released Glycan Analysis of Trastuzumab Biosimilar using LCMS-9030

Monoclonal antibodies (mAbs) are a major class of biopharmaceuticals with indications now covering a large panel of diseases, from cancer to asthma, including central nervous system disorders, infectious diseases, and cardiovascular diseases. Glycosylation is a very commonly occurring posttranslational modification (PTM) in mAbs that plays a vital role in the safety and efficacy of many therapeutic mAbs.

Puranik, A., Jain, R., Tupe, D., Rasam, P., Goswami, R. (2023) Shimadzu Document No. 06-SAIP-LC-059-EN

A Unique Workflow for Glycoprotein Characterization from Sample Preparation to MS/MS Spectral Interpretation

Protein glycosylation is a complex dynamic post-translational modification, which is used by an organism to regulate a number of important protein function.

Albanese, J., Lee, R. (2011)
J Biomol Tech., 22(Suppl), S57

SimGlycan™ Software*: A New Predictive Carbohydrate Analysis Tool for MS/MS Data Automated Data Interpretation for Glycan Characterization

The analysis of carbohydrates is of great importance in modern biochemistry and is often tackled using various analytical techniques, including mass spectrometry.

Albanese, J., Glueckmann M., Lenz C. (2010)

Novel Glycan Column Technology for the LC-MS Analysis of Labeled and Native N-Glycans Released from Proteins and Antibodies

Because glycosylation is critical to the efficacy of antibody therapeutics, the FDA requires that a consistent human-type glycosylation be maintained for recombinant monoclonal antibodies (mAb), irrespective of the system in which they are produced.

Aich, U., Birznieks, I., Saba, J., Liu, X., Viner, R., Hao, Z., Gendeh, G.S., Rao, S., Huhmer, A., Agroskin, Y., Pohl, C. (2013)
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Resources

Literature

MultiNotch MS3 Approach for Relative Quantification of N-Glycans Using Multiplexed Carbonyl-Reactive Isobaric Tags...

Creating a Mass Spectral Reference Library for Oligosaccharides in Human Milk...

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Posters

Automated and Rapid Characterization of Low-Molecular-Weight Heparin Oligosaccharides Using TripleTOF 5600 Mass Spectrometer...

SimGlycan: Informatics Support for Automated Characterization of Low-Molecular-Weight...

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Application Notes

A Unique Workflow for Glycoprotein Characterization from Sample Preparation to MS/MS Spectral...

SimGlycan Software: A New Predictive Carbohydrate Analysis Tool for MS/MS Data Automated Data Interpretation for Glycan Characterization...

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Webinar

Automating mass spectrometry based glycan identification and quantitation using SimGlycan software

Use SimGlycan for glycan and glycopeptide analysis, glycan structure prediction, glycosylation analysis...

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