NetPrimer analyzes primers to optimize PCR, sequencing, and hybridization reactions. Primers are analyzed for amplification related properties including melting temperature (Tm) and secondary structures.
The program has been designed to be easy to use. To analyze a primer, just type in the primer sequence and click the Analyze button. All of the properties of the primer are displayed. To analyze a primer pair, use the sense/anti-sense radiobutton to enter both primers. Cross dimers are now calculated in addition to the properties for each primer.
A primer report can be printed containing detailed information on the primer pair. Primer name and description areas are provided to aid in identification of the primer from the printout.